http://13.232.72.61:8080/jspui/handle/123456789/206 2026-04-04T03:01:42Z http://13.232.72.61:8080/jspui/handle/123456789/6646 Title: Method Development and Validation for Simultaneous Estimation of Losartan and Ramipril in Marketed Formulations Authors: Sindhura, T; Cendil Kumar, A Abstract: A simple, fast and precise reversed phase high pressure liquid chromatography method has been developed and validated for the simultaneous estimation of Losartan and Ramipril in marketed formulations (tablets). Phenomenex Luna C18, 5 μ, 250 × 4.6 mm I.D column with mobile phase Acetonitrile: Methanol: o-phosphoric acid (60:40:1%) were used. The flow rate was 1.0 ml/min and effluent was monitored by UV/Vis detection at 254 nm. The proposed method was validated for parameters viz., Specificity, Accuracy, Precision, Linearity, Range, LOD, LOQ, and System Suitability. The linearity of Losartan and Ramipril were in the range of 5-25 μg/ml respectively. The percentage recovery was found to be 98.3-100.9% and 99.42- 101.5% for Losartan and Ramipril respectively. The proposed method can be used for the routine analysis of the drugs in bulk and in dosage form. Description: Dissertation 2011-05-01T00:00:00Z http://13.232.72.61:8080/jspui/handle/123456789/6645 Title: Method Development and Validation for Simultaneous Estimation of Aspirin and Dipyridamole in Marketed Formulations Authors: Govardhan, G; Cendil Kumar, A Abstract: A simple, fast and precise reversed phase high pressure liquid chromatography method has been developed and validated for the simultaneous estimation of Aspirin and Dipyridamole in marketed formulations (Capsules). Phenomenex Luna C18, 5 μm, 250 × 4.6 mm I.D. column with mobile phase acetonitrile: water (60:40 v/v), flow rate 1.0 ml/min and the effluent monitored by UV detection at 230 nm. The proposed method was validated for parameters viz., specificity, accuracy, precision, linearity, range, limit of detection, limit of quantitation, system suitability. The linearity of Aspirin and Dipyridamole were in the range of 5-25 μg/ml respectively. The percentage recovery was found to be 98.16- 101.2% and 98.75-99.6% for Aspirin and Dipyridamole respectively. The proposed method can be used for the routine analysis of the drugs combination in dosage form and in bulk powders. Description: Dissertation 2011-05-01T00:00:00Z http://13.232.72.61:8080/jspui/handle/123456789/6644 Title: Method development and validation for simultaneous estimation of Mefenamic acid and Paracetamol in marketed formulations by instrumental method Authors: Ritesh R., Patel; Narajji, Chandrashekhar Abstract: A RP-HPLC and UV-visible spectrophotometric for simultaneous estimation of Mefenamic acid and Paracetamol in marketed tablet formulation were developed and validated. A RP-HPLC method was developed for simultaneous estimation of Mefenamic acid and Paracetamol tablet formulation using Phenomenex Luna C18 column (250 mm × 4.6 mm id, 5 μm particle size) and a mobile phase of acetonitrile : water (70:30 v/v), at flow rate 1.0 ml/min with UV detection at 268 nm. The retention time (tR) of Mefenamic acid and Paracetamol found to be 4.09 and 2.66 min respectively. The proposed method was validated for system suitability, specificity, linearity, accuracy, precision, LOD, LOQ and robustness. All parameters were found to be within the acceptance limit. Linearity over the concentration range 5-30 μg/ml for both Mefenamic acid and Paracetamol with regression coefficient (r2) 0.9999 and 0.9999 respectively. Limit of detection (LOD) found to be 0.0059 μg/ml and 0.008 μg/ml whereas limit of quantitation (LOQ) found to be 0.018 μg/ml and 0.026 μg/ml for Mefenamic acid and Paracetamol respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.96%-100.62% and 99.12%-100.2% for Mefenamic acid and Paracetamol respectively. A UV-visible spectrophotometric method was developed for simultaneous estimation of Mefenamic acid and Paracetamol in marketed tablet formulation using methanol as solvent. The method involved Q-absorbance ratio method based on the measurement of absorbance at two wavelengths, i.e. λmax of Paracetamol (248 nm) and iso-absorptive point of both drugs (268 nm). Linearity found over the concentration range of 2-10 μg/ml for both Mefenamic acid and Paracetamol with regression coefficient (r2) 0.9991 and 0.9995 respectively at 248 nm and 0.9992 at 268 nm. LOQ for Mefenamic acid and Paracetamol found to be 0.33 μg/ml and 0.10 μg/ml at 248 nm respectively and 0.28 at 268 nm whereas LOD found to be 0.11 μg/ml and 0.034 μg/ml at 248 nm respectively and 0.092 at 268 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. All parameters were found to be within the acceptance limit. The accuracy of the proposed method was determined by recovery studies and found to be 100.56%-102.5% and 100.22%-101.03% for Mefenamic acid and Paracetamol respectively. The proposed methods are simple, accurate, precise and suitable for analysis of marketed tablet formulation containing Mefenamic acid and Paracetamol Description: Dissertation 2011-05-01T00:00:00Z http://13.232.72.61:8080/jspui/handle/123456789/6643 Title: Method development and validation for simultaneous estimation of Diacerein and Glucosamine in marketed product Authors: Akbar G., Momin Mohammad; Narajji, Chandrashekhar Abstract: A RP-HPLC method was developed for simultaneous estimation of Diacerein and Glucosamine tablet formulation using Phenomenex Luna C18 column (250 × 4.6 mm i.d., 5 μm particle size) and a mobile phase of acetonitrile: o-phosphoric acid solution (0.001 % v/v) in HPLC water pH 4 (40:60 v/v), at flow rate 1.0 ml/min with UV detection at 220 nm. The retention time (tR) of Diacerein and Glucosamine found to be 15.449 and 1.996 min respectively. The proposed method was validated for linearity, accuracy, precision, LOD, LOQ. Linearity, accuracy and precision were found to be well within the acceptance limit. Linearity over the concentration range found to be 5-25 μg/ml for Diacerein and 100-500 μg/ml for Glucosamine with regression coefficient (r2) 0.9994 and 0.9992 respectively. Limit of detection found to be 0.52 and 0.16 μg/ml whereas limit of quantition found to be 1.6 and 0.509 μg/ml for Diacerein and Glucosamine respectively. Precision calculated by % RSD for Diacerein 0.15-1.5 and Glucosamine 0.02-0.8 %. The accuracy of the proposed method was determined by recovery studies and found to be 99.25-99.98 and 99.47-100.51 % Diacerein and Glucosamine respectively. A UV-visible spectrophotometric method was developed for simultaneous estimation of Diacerein and Glucosamine in marketed tablet formulation using HPLC methanol as solvent. The proposed method was developed by simultaneous equation method and absorbencies were measured at wavelength 257 nm for Diacerein and 202 nm for Glucosamine. Linearity over the concentration range found to be 2-10 and 25-100 μg/ml respectively with regression coefficient (r2) 0.9991 and 0.9993 respectively. LOD found to be 0.025 and 1.031μg/ml respectively whereas LOQ found to be 0.076 and 3.125 Abstract Department of Quality Assurance, ABMRCP, Bangalore-90 μg/ml for Diacerein and Glucosamine respectively. Precision calculated by % RSD for Diacerein 0.21-1.2 and Glucosamine 0.8-1.23 %. The accuracy of the proposed method was determined by recovery studies and found to be 102.66-110.19 and 102.16-102.89 % for Diacerein and Glucosamine respectively. The proposed methods are simple, accurate, precise and suitable for analysis of marketed tablet formulation containing Diacerein and Glucosamine. Description: Dissertation 2011-05-01T00:00:00Z