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Study on Cerebroprotective action of Hesperetin against cerebral ischemia-reperfusion induced cerebral infarction in both normal and Streptozotocin-induced Diabetic Rats

Tue, 01 May 2012 00:00:00 GMT

Title: Study on Cerebroprotective action of Hesperetin against cerebral ischemia-reperfusion induced cerebral infarction in both normal and Streptozotocin-induced Diabetic Rats Authors: Hiremath, Sidramesh; Ansari, Mohammad Asif Abstract: Aim: To investigate cerebroprotective action of Hesperetin against cerebral ischemiareperfusion induced cerebral infarction in both normal and streptozotocin-induced diabetic Rats. Methods: In the present study, male wistar albino rats weighing 150-250 gm were used. Rats were anesthetized by giving thiopentone sodium (45 mg/kg) by i.p. earlier published method was adopted for inducing cerebral ischemia. Ischemic condition was achieved by BCCAO method for 30 min ischemic, followed by 48 h reperfusion. The allopurinol (10 mg/kg) (XO inhibitor), L-NAME (10 mg/kg) (NO inhibitor) and nimesulide (20 mg/kg) (COX inhibitor) were administered 10 min before ischemia and hesperetin (30 mg/kg) was administered 10 min before reperfusion period. Then after 48 h reperfusion, animals were sacrificed and immediately brain was removed, homogenized, centrifuged and supernatant was collected, then various enzyme estimations were done and same procedure was followed in STZ (45 mg/kg; i.p.) induced diabetic rats. Results: In I/R group showed significant increase in malondialdehyde, myeloperoxidase and depletion in catalase and superoxide dismutase levels was observed. Combined treatment of hesperetin with allopurinol significantly decreased the MDA and MPO levels where as increased the SOD and CAT levels when compared I/R group in both non-diabetic and diabetic rats. Rare, cerebroprotection was offered by hesperetin when given along with nimesulide. Where as, Hesperetin failed to show synergestic activity with L-NAME. Conclusion: These findings suggest the cerebral injury due to over production of free radicals was inhibited by combined treatment of hesperetin and allopurinol by limiting the XO pathway that suggests antioxidant property. Description: Dissertation

Estimation of TNF-α, TGF-α and p53 for the anticancer activity of Basella rubra Linn. leaves on HL-60 cell lines

Tue, 01 May 2012 00:00:00 GMT

Title: Estimation of TNF-α, TGF-α and p53 for the anticancer activity of Basella rubra Linn. leaves on HL-60 cell lines Authors: Devineni, Satya Prakash; Divakar, Kalyani Abstract: Background & Objectives: Cancer is a general term used frequently to indicate various types of malignant neoplasms, most of which invade surrounding tissue and may metastasize to several sites. Many Ayurvedic herbal drugs have been listed with possible antineoplastic activity. Sadafuli (Catharanthus roseus) – vinblastin, vincristin; Talispatra (Taxus bravifolia) – Taxol; are few examples of plant based anticancer drugs. Basella rubra Linn. is one of the edible herbs among list, on which very less research work i.e. antiulcer, antifungal, target for HIV activities have been reported and the earlier studies in our laboratory on the leaves of Basella rubra Linn. has showed the anticancer activity. Hence, the present work is to explore the mechanism involved in the anticancer activity of leaves of Basella rubra Linn. Methods: HL-60 (Human promyelocytic leukemia) cell lines were obtained from NCCS, Pune and were cultured in RPMI-1640 and DMEM growth medium respectively supplemented with 10% FBS. The cytotoxic and antiproliferative effect of Basella rubra Linn. in the doses of 6.12- 200 μg/ml on HL-60 cancer cells were determined by MTT assay, Clonogenic assay and DNA fragmentation assay was observed using FACS analysis. HAEBR was used in the doses of 100- 1000 μg/ml for estimating the levels of TNF-, TGF- and p53. Results: The maximum increase in the levels of TNF- and p53 was observed with HAEBR (1000 μg/ml) and the level of TGF- significantly decreased in HL-60 cell lines compared to the control group. HAEBR showed significant (P<0.05) dose dependent decrease in percentage cell viability on HL-60 cell line as compared to the control in MTT assay. HAEBR produced very significant (P<0.001) dose dependent decrease in percentage colony growth on HL-60 cell line as compared to control and also caused DNA fragmentation in dose dependent manner. Conclusion: The present study demonstrated that the hydro-alcoholic extract of leaves of Basella rubra Linn. possesses anticancer activity. This effect has been mediated by increasing the levels of TNF-, p53 and decreasing the level of TGF-. Description: Dissertation

Prophylactic antidiabetic effect of Amlaras and Lycopene alone and in combination in rats

Tue, 01 May 2012 00:00:00 GMT

Title: Prophylactic antidiabetic effect of Amlaras and Lycopene alone and in combination in rats Authors: S, Zabiulla; Divakar, Kalyani Abstract: Aim: Prophylactic antidiabetic effect of Amlaras and Lycopene alone and in combination in rats Methods: In the present study, male Wistar albino rats of weighing 150-250 gm were procured from authorized suppliers. Rats were anesthetized light by giving di-ethyl ether. Blood is withdrawn from retro-orbital plexus and cold centrifuged for blood serum. The drugs Amlaras (500 mg/kg; p.o.) and Lycopene (4 mg/kg; p.o.) were administered for 14-days and on 14th day blood serum was collected and estimated for following parameters like SOD, GSH, LPO and Glucose. On 15th day, diabetes was injected by means of Alloxan (110 mg/kg; i.p.) and STZ (45 mg/kg; i.p.). The blood serum was collected and estimated on 1, 3rd and 7th day of diabetes. Results: There was significant increase in the antioxidant levels like GSH, SOD and NO and decrease in LPO in blood serum glucose level compared to their respective control group and also shown significant synergistic effect in combination. Conclusion: These findings suggest that Amlaras and Lycopene may have prophylactic effect in diabetes and also have synergistic effect in combination in both Alloxan and STZ induced diabetic rats. Keywords: Prophylaxis, Amlaras, Lycopene and Oxidative stress. Description: Dissertation

Cardioprotective Effect of Bioflavonoids Quercetin & Catechin in Isoproterenol Induced Myocardial Infarction

Tue, 01 May 2012 00:00:00 GMT

Title: Cardioprotective Effect of Bioflavonoids Quercetin & Catechin in Isoproterenol Induced Myocardial Infarction Authors: Swathi, K; P M, Manjunatha Abstract: Myocardial infarction is making an increasingly important contribution to mortality statistics of CVD. The present study was aimed to investigate the cardioprotective effect of Quercetin and Catechin in isoproterenol induced myocardial infarcted rats. Subcutaneous injection of isoproterenol (150 mg kg-1) to rats at an interval of 24 h for 2 days showed a significant (p<0.001) increase in cardiac marker enzymes (LDH, SGOT and SGPT) in serum, increase in MDA levels in both tissue and serum; significant decrease in antioxidant enzymes (SOD,GPX and CAT). Pretreatment with Quercetin (30 and 60 mg kg-1, o. p.) and Catechin (20 and 40 mg kg-1, o. p.) to isoproterenol treated rats for a period of 42 days showed significant (p<0.001) protective effect. Administrations of Quercetin and Catechin perse to normal rats have shown very less significant effect on above parameters. So the study revealed that the cadioprotective effect of Quercetin and Catechin in isoproterenol induced myocardial infarction may be due to an attenuation of antioxidant enzymes and inhibition of lipid peroxidation of membrane and decrease in the levels of cardiac markers, which was further confirmed by histopathological studies. Description: Dissertation