http://13.232.72.61:8080/jspui/handle/123456789/6223 Sat, 04 Apr 2026 04:25:38 GMT 2026-04-04T04:25:38Z http://13.232.72.61:8080/jspui/handle/123456789/6274 Title: Optimization of process parameters for alkaline protease production under solid-state fermentation by Thermoactinomyces thalpophilus PEE 14 Authors: Goli, Divakar; Sunitha, M; Vasu, P; Shanker, UP; Ellaiah, P Abstract: Production parameters of extracellular alkaline protease employing our laboratory isolate Thermoactinomyces thalpophilus PEE 14 under solid-state fermentation (SSF) were optimized. Wheat bran the best substrate among the 15 substrates used for optimization, showed the highest activity (1620 PU/g). The physical and chemical parameters were also optimized. The maximum enzyme activity under optimum conditions was obtained with incubation period 72 h, incubation temperature 55ºC, initial pH 10, inoculum level 20%, level of salt solution 2:10 and initial moisture level 80%. Increase of enzyme activity by 60% (2576 PU/g) was observed when compared with the unoptimized conditions. Sun, 01 Jan 2006 00:00:00 GMT http://13.232.72.61:8080/jspui/handle/123456789/6274 2006-01-01T00:00:00Z http://13.232.72.61:8080/jspui/handle/123456789/6229 Title: Studies on process and nutritional parameters for production of alkaline protease by Thermoactinomyces thalpophilus PEE 14 Authors: Ellaiah, P; Goli, Divakar; Vasu, P; Sunitha, M; Shankar, P Uday Abstract: Studies on process and nutritional parameters for the production of extracellular alkaline protease employing Thermoactinomyces thalpophilus PEE 14 was carried out. Different initial pH, incubation temperatures and time and inoculum levels and their age were studied. Effects of different nutritional parameters, trace elements and metabolic inhibitors on protease production were also studied. The maximum enzyme activity was obtained with incubation temperature of 55ºC, initial pH of 10, incubation time of 24 h, level of inoculum 10 per cent and age of the inoculum 72 h. Results of nutritional and trace element parameters showed that pyridoxin, L-cysteine and sodium molybdate showed best vitamin, amino acid and trace elements, respectively. Silver nitrate, antibiotic framycitine and surfactant cetrimide showed strong inhibitory effect on protease production. Sat, 01 Oct 2005 00:00:00 GMT http://13.232.72.61:8080/jspui/handle/123456789/6229 2005-10-01T00:00:00Z