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Nanopaticulate Drug Delivery System for Anti-Cancer Drugs

Thu, 01 Oct 2020 00:00:00 GMT

Title: Nanopaticulate Drug Delivery System for Anti-Cancer Drugs Authors: Tajmal, Mandana; Goli, Divakar Abstract: An attempt was made in this study to formulate and evaluate the polymeric nanoparticles of anti-cancer drug using biodegradable and biocompatible polymers such as chitosan and PLGA. In this formulation Gefitinib was used as model drug because of its wide range of use in treatment of cancer such as non-small cell lung cancer, breast cancer, pancreatic cancer etc., in the form of tablet. FTIR and DSC confirmed that there is no interaction between the drug and polymers and excipients used in the formulations. Gefitinib loaded chitosan nanoparticles were prepared using ionic gelation technique and use of different concentrations of chitosan and STPP was used as cross linker. Gefitinib loaded PLGA nanoparticles were prepared by means of nanoprecipitation method and emulsification followed by homogenization technique using different concentrations of PLGA and stabilized by PVA. The prepared nanoparticles were characterized in case of %entrapment efficiency, particle size, poly dispersity index, zeta potential, %release upto 72 h. After response optimization study, GCN1 and A5 were selected as optimized formulations. Morphological characterization by SEM, stability studies, in-vitro cytotoxicity studies on A549 cell lines by MTT assay and in-vivo anti-cancer effect on non-small cell lung cancer induced nude mice were done for optimized formulations. Effect of the prepared nanoparticles were evaluated for body weight change, blood hematological parameters, mean survival time and %increase in life span and compared with Gefitinib. A5 and GCN1 showed 29.16% and 20.83% increase in life span as compared with Gefitinib which showed 16.66%. Thus the study revealed that the developed nanopaticulate system has a great appeal for the conventional treatment for non-small cell lung cancer. Description: Theses

Design and evaluation of drug loaded polymeric nanoparticles of antibacterial agent for the treatment of pneumonia

Tue, 01 May 2018 00:00:00 GMT

Title: Design and evaluation of drug loaded polymeric nanoparticles of antibacterial agent for the treatment of pneumonia Authors: H M, Ashvini; Kalyanap, Shivanand Abstract: Aim: The current research was aimed at formulating and optimizing polymeric nanoparticles of clarithromycin (an antibacterial agent) in terms of particle size, entrapment efficiency, in vitro release behaviors and antibacterial efficiency against S. Pneumoniea. Methods: Clarithromycin loaded polymeric nanoparticles are prepared using chitosan (low molecular weight) by ionic gelation method and Poly (lactide –co-glycolic acid) (50:50 monomer ratio) by emulsification solvent evaporation and nanoprecepitation method. Tripolyphosphate used as a cross linker and polyvinyl alcohol, poloxamer- 188 as stabilizers. Carbapol, Poloxmer-188 and Tween 80 were added as co-stabilizer and co-surfactant respectively. Clarithromycin –β-cyclodextrin complex was prepared and incorporated into chitosan nanoparticles. Minitab 17 statistical software was employed to create general full factorial design. NPs were characterized in terms of surface morphology, particle size and distribution, zeta potential, encapsulation efficiency, clarithromycin release profile and antibacterial activity. The antibacterial activity of nanoparticles against S. Pneumoniea was evaluated by calculation of minimum inhibitory concentration and zone of inhibition. Drug-excipient compatibility study by FTIR and DSC revealed no possible interactions. Description: Theses

Nanoparticulate mediated targeted drug delivery system of anti-Alzheimer’s drug;Nanoparticulate mediated targeted drug delivery system of anti-Alzheimer’s drug;

Tue, 01 Mar 2016 00:00:00 GMT

Title: Nanoparticulate mediated targeted drug delivery system of anti-Alzheimer’s drug;Nanoparticulate mediated targeted drug delivery system of anti-Alzheimer’s drug; Authors: Patel, Kinjal Chetankumar; Goli, Divakar Abstract: In present study, we aimed to formulate RT loaded nanoparticles, which will give the best result for improvement of memory, cognitive deficits and prevention against degeneration of hippocampus in Alzheimer’s disease. We prepared two types of nanoparticles: Polymeric and Solid lipid nanoparticles. Polymeric nanoparticles PLGA-CS-Tween 80, PLGA-CS-PEGs and PLGA-Soya lecithin-Tween 80 were prepared by emulsification-solvent evaporation, oil-in-water (O/W) single emulsion solvent evaporation method, modified nanoprecipitation technique combined with self assembly respectively. Solid lipid nanoparticles were prepared by modified cold homogenization method. The nanoparticles were evaluated for FTIR, DSC, particle size, polydispersity, zetapotential, in vitro drug release, stability study, SEM, behavioral study and histopathology of hippocampus. The FTIR and DSC study demonstrated that there was no interaction between drug, polymers and lipids, which indicated that they are compatible with each other. Using factorial design for optimization we tried to achieve higher entrapment efficiency and drug release with smaller particle size (<200 nm) and narrow polydispersity index, with less number of experiments. The SEM study showed that particles were spherical in shape with smooth/rough surface. The stability study for six months demonstrated that the formulations were stable at refrigerator (3-5 °C) condition, hence is the most suitable condition for the storage of nanoparticles. Administration of optimized formulations of polymeric and solid lipid nanoparticles in Aluminium chloride induced Alzheimer’s disease model (Wistar rats) results in noticeable improvement in learning and memory capacity and it antagonized the toxic effect of Aluminium chloride by reduction in escape latency compared to standard drug solution treated animals. Description: Theses

Bioparametric investigation on the production of alkaline protease by a newly isolated thermoactinomyces thalpophilus PE-120

Wed, 01 Jan 2003 00:00:00 GMT

Title: Bioparametric investigation on the production of alkaline protease by a newly isolated thermoactinomyces thalpophilus PE-120 Authors: Goli, Divakar; P, Ellaiah Abstract: Bioparametric investigation on the production of alkaline protease by a newly isolated thermoactinomyces thalpophilus PE-120 Description: Theses