Anti HIV and Antibacterial property of Coumarinsisolated from Sonchus oleraceus

Abstract

The acquired immunodeficiency syndrome (AIDS) is a result of human immunodeficiency virus (HIV) infection which leads to severe suppression of immune functions. Numerous plant derived compounds have been found to inhibit different steps in the HIV replication cycle. Sonchus oleraceus is belongs to Asteraceae family have some medicinal property. The plant is a rich source of many biotic compounds which are of medicinal importance. The study was undertaken to investigate the anti-HIV and anti-bacterial activities of coumarin compounds extracted from the Sonchus oleraceus. Microwave assisted extraction method is followed for the extraction process using three solvents i.e. ethanol, methanol and water at different temperature for 5 min followed by one or two cycle extraction method. The obtained extracts were subjected to identification of coumarin using three different methods via, FeCl3, fluorescence method and other coumarin test. Extracts were also subjected to phytochemical analysis to check the presence of other phytochemical compounds. Screening of coumarin compound for the inhibitory action against protease enzymes involve in the replication of HIV.The methanol extracts of Sonchus oleraceushave shown significant inhibition of protease activity as compared to standard (lopinavir).