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http://13.232.72.61:8080/jspui/handle/123456789/2245| Title: | Isolation and Identification of Cellulose Degrading Microbes |
| Authors: | Shareef, Ismail Gopinath, S. M Satheesh, Manasa Christopher, Samuel Xavier |
| Keywords: | Biotechnology Bacillus subtilis Aspergillusniger CMC assay Protein assay Cellulose |
| Issue Date: | Aug-2015 |
| Publisher: | International Journal of Innovative Research in Science, Engineering and Technology |
| Citation: | Shareef, Ismail., Satheesh, Manasa., & Christopher, Samuel Xavier. (2015). Isolation and Identification of Cellulose DegradiMicrobes. International Journal of Innovative Research in Science, Engineering and Technology, 8(4), 6788-6793. |
| Abstract: | Cellulose degrading bacteria was isolated from soil where as cellulose degrading fungi was isolated from soil and decaying wood. The bacteria were identified as Bacillus subtilis and the fungi were identified as Aspergillusniger and Trichodermaviride. Their cellulase degrading ability was screened by Congo red plate assay. Their enzyme activity was tested with CMC assay, and the protein estimation was done by Lowry’s method. Aspergillusniger showed the highest cellulase activity of 1861.611IU/ml, followed by Trichodermaviride 780.676IU/ml and Bacillus subtilis showing the least cellulase activity of 450.389IU/ml. Protein estimation was also carried out, in which Aspergillusniger showed 0.18 mg/ml, Trichodermaviride showed 0.15mg/ml and Bacillus subtilis showed 0.31mg/ml. |
| URI: | http://13.232.72.61:8080/jspui/handle/123456789/2245 |
| ISSN: | e-2319-8753 p-2347-6710 |
| Appears in Collections: | Faculty Publications |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Isolation and Identification of Cellulose Degrading Microbes.pdf | 694.92 kB | Adobe PDF | View/Open |
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