Anti cancer effect of aqueous extract of Gmelina arborea roxb leaves

Abstract

Background & Objectives: Gmelina arborea roxb commonly known as ‘Gambhari’ tree, the various parts of the plants are widely used in diarrhoea, anti-pyretic, thirst, anemia, leprosy, ulcers, consumption, strangury, vaginal discharges. Cancer is one of the major causes of death. It may affect people at all ages, even fetuses, but the risk for most varieties increases with age. Cancer causes about 13% of all deaths. Chemotherapy is a major treatment modality for cancer. Most of the chemoterapic agents exhibit several normal tissue toxicity accompanied by undesirable side effects and many of the potent antineoplastic drugs are highly expensive, mutagenic, carcinogenic and teratogenic. Herbal medicine is inexpensive and can be affordable by all the section of society. The objective of the present study is to evaluate the anti cancer effect of aqueous extract of leaves of Gmelina arborea roxb (AEGA). Methods: The anti cancer effect of AEGA was evaluated by using different experimental models such as micronucleus test (using mice bone marrow), Ames assay (using Salmonella typhimurium), MTT assay, Cell viability assay, DNA fragmentation and clonogenic assay (using HL-60cells). AEGA were used in the doses of 286 mg/kg and 667 mg/kg on micronucleus test and 5, 10, 15 and 20 mg/ml in Ames assay, MTT assay, Cell viability assay, DNA fragmentation and clonogenic assay.