Isolation and Characterization of Pentadecanoic Acid Ethyl Ester from the Methanolic Extract of the Aerial Parts of Anisomele Smalabarica R.Br

Abstract

Anisomeles malabarica (L). R.Br., (Lamiaceae) is distributed in major parts of India, especially in South India it is known as a traditional medicinal plant reported to possess anti-spasmodic, antiinflammatory properties and is used in Rheumatoid arthritis. The preliminary phytochemical investigation on the methanolic extract of the aerial parts of the plant revealed the presence of carbohydrates, phytosterols and triterpenoids. The aim of the current study was to isolate and characterize the bioactive compounds from the aerial parts of Anisomeles malabarica as the plant is reported to possess potent anti-inflammatory properties and is also used in the treatment of Rheumatism. For isolation purpose, the dried powder of was extracted with methanol using Soxhlet apparatus continuously for 16 hours. The extract was dried under reduced pressure to evaporate the solvent and the dried mass was taken for the isolation work. Pentadecanoic acid ethyl ester was isolated by column chromatography from the methanolic extract of aerial parts of Anisomeles malabarica. The structural elucidation of the isolated compound was on the basis of spectroscopic analysis.